Probiotics HU36®
Bacillus indicus (HU36®)

Bacillus indicus HU36® offers a new paradigm to present to a very competitive probiotic market place. The notion that beneficial bacteria also have the role of digesting foods we eat and in turn, producing nutritious compounds for our bodies to absorb, is the next evolution in the dietary-supplement market. Bacillus HU36®, being a spore, offers advantages of better colonization and survival through the digestive track when compared to non-spore probiotic organisms; it also offers claims related to the most bioavailable form of antioxidants and carotenoids.

Bacillus indicus HU36® (Bacillus HU36®) is a unique patented strain of gram-positive spore forming bacterium that produces a distinct yellow-orange pigmentation. The pigmentation is due to the synthesis of carotenoids, which are gastric stable, bio-accessible, and significantly more bioavailable than carotenoids from other sources.

What makes HU36® Unique
  • Human origin
  • Can survive the harsh conditions associated with food processing and may be incorporated into a range of food matrices without the need for refrigeration.
  • Ability to withstand heating at 235ᵒC up to 8 minutes, which allows for incorporation into a number of cooked and baked food products.
  • Extremely stable in the acidic conditions of the stomach thus reaches the intestine intact and ensures high bioavailability.
  • Also uniquely stable in liquids at room temperature and will remain dormant in non-refrigerated beverages.
  • Bacillus indicus HU36® produces carotenoids that are more bioaccessible& bioavailable (about 4.5 times as high) than β-carotene. The carotenoids consist of keto/hydroxyl-carotene derivatives, astaxanthin, 4-etozeaxanthin, echinenone, a hydroxylechinenone, derivative, phenicoaxanthin, canthaxanthin, zeaxanthin, and/or α-carotene.
  • Bacillus indicus HU36® carotenoids have superior antioxidant activity compared to lycopene.
Probiotics HU36®

The Benefits Of HU36®

  • Gastric stability: They survive transit through the GI-tract unlike other commercial carotenoid supplements that are rapidly degraded when ingested.
  • Heat stability: As spores, Bacillus strains that produce carotenoids can be stored indefinitely. This enables them to be incorporated into food matrices, a process not possible with other sources of carotenoids. Further, spores can survive pasteurization and short-term exposure to high temperatures of baking. The spores produce these highly bioavailable carotenoids in the gut itself where absorption rates are quite high.
  • Bioavailability: Bacillus indicus HU36® carotenoids appear to be absorbed in the GI-tract. These pre-publication results arise from studies conducted by the Colorspore project and demonstrate the potential of Bacillus indicus HU36® carotenoids. This is a key aspect of the project and necessary to fully sustantiate health claims. The studies also indicate that Bacillus indicus HU36® carotenoids are more stable, under physiological conditions, that standard purified products.
  • Antioxidant acidity: Bacillus indicus HU36® carotenoids have superior antioxidant activity compared to lycopene. Activity is shown in both hydrophilic and hydrophobic phases promising potential health benefits and applications. Bacillus indicus HU36® carotenoids were shown to be more powerful antioxidants under physiological conditions.
  • Existing use as food supplement: Bacillus spores are in widespread use as probiotic supplements. They have attracted growing interest as probiotics and have a number of health related benefits acribed to them in vitro and also in vivo including human studies. Conceptually, at least, this may support their use as food supplements.
  • Safety: Bacillus indicus HU36® has been subjected to toxicology studies and no adverse effects have been determined. The Bacillus indicus HU36® carotenoids have a long history of human exposure as the strain is a human isolate. This has a favorable safety profile over synthetic carotenoids.
  • Potential health benefits: The carotenoids found in Bacillus indicus HU36® are apolycopene derivatives and the same class that has been shown to play a role in reducing the risk of certain cancers, notably prostate cancer.
  • Production: Bacterial spore production is commercially viable as demontrated by the number of companies that produce them as probiotics for food and supplement applications. Bacillus indicus HU36® offers a live bacterial product that is straightforward and attractive, operationally, in contrast to purifying or synthesizing carotenoids. In addition, Bacillus indicus HU36® is the only ingredient on the market that can penetrate the probiotic and antioxidant markets with a single and cost effective ingredient. Based on the nutritional benefits of Bacillus indicus HU36®, matching its value by mixing a standard spore forming probiotic strain with purified carotenoids would prove to be a cost barrier.


HU36® is a Bacillus indicus strain isolated from the gastro-intestinal tract of healthy humans.